Spectroscopic and kinetic studies of Nor1, a cytochrome P450 nitric oxide reductase from the fungal pathogen Histoplasma capsulatum
نویسندگان
چکیده
منابع مشابه
Phylogeography of the fungal pathogen Histoplasma capsulatum.
Until recently, Histoplasma capsulatum was believed to harbour three varieties, var. capsulatum (chiefly a New World human pathogen), var. duboisii (an African human pathogen) and var. farciminosum (an Old World horse pathogen), which varied in clinical manifestations and geographical distribution. We analysed the phylogenetic relationships of 137 individuals representing the three varieties fr...
متن کاملFungal denitrification and nitric oxide reductase cytochrome P450nor.
We have shown that many fungi (eukaryotes) exhibit distinct denitrifying activities, although occurrence of denitrification was previously thought to be restricted to bacteria (prokaryotes), and have characterized the fungal denitrification system. It comprises NirK (copper-containing nitrite reductase) and P450nor (a cytochrome P450 nitric oxide (NO) reductase (Nor)) to reduce nitrite to nitro...
متن کاملCharacterization of the mechanism of cytochrome P450 reductase-cytochrome P450-mediated nitric oxide and nitrosothiol generation from organic nitrates.
Mammalian cytochrome P450 reductase (CPR) and cytochrome P450 (CP) play important roles in organic nitrate bioactivation; however, the mechanism by which they convert organic nitrate to NO remains unknown. Questions remain regarding the initial precursor of NO that serves to link organic nitrate to the activation of soluble guanylyl cyclase (sGC). To characterize the mechanism of CPR-CP-mediate...
متن کاملIdentifying phase-specific genes in the fungal pathogen Histoplasma capsulatum using a genomic shotgun microarray.
A fundamental feature of the fungal pathogen Histoplasma capsulatum is its ability to shift from a mycelial phase in the soil to a yeast phase in its human host. Each form plays a critical role in infection and disease, but little is understood about how these two morphologic phases are established and maintained. To identify phase-regulated genes of H. capsulatum, we carried out expression ana...
متن کاملImmunological studies on Histoplasma capsulatum.
Alveolar macrophages freshly harvested from normal and immunized rabbits were parasitized with yeast cells and protoplasts of Histoplasma capsulatum. Macrophages obtained from either normal or sensitized rabbits failed to phagocytize protoplasts, whereas, the yeast cells were actively ingested. There was no detectable intracellular killing by macrophages. A serological similarity was found betw...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 2008
ISSN: 0003-9861
DOI: 10.1016/j.abb.2008.09.001